Engineered sucrose phosphorylase variant enzymes
AssigneeCodexis, Inc.
InventorsJonathan Vroom, Santhosh Sivaramakrishnan, Nandhitha Subramanian
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Abstract
The present invention provides engineered sucrose phosphorylase (SP) enzymes, polypeptides having SP activity, and polynucleotides encoding these enzymes, as well as vectors and host cells comprising these polynucleotides and polypeptides. Methods for producing SP enzymes are also provided. The present invention further provides compositions comprising the SP enzymes and methods of using the engineered SP enzymes. The present invention finds particular use in the production of pharmaceutical compounds.
Claims21
An engineered sucrose phosphorylase comprising a polypeptide sequence having at least 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NO:
- 2, wherein the polypeptide sequence of said engineered sucrose phosphorylase comprises at least one substitution at position 397 and wherein the amino acid positions of said polypeptide sequence are numbered with reference to SEQ ID NO:
- 2, and wherein the engineered sucrose phosphorylase exhibits improved activity on a substrate selected from the group consisting of sucrose, sucrose-related disaccharides and/or inorganic phosphate as compared to wild-type Alloscardovia omnicolens sucrose phosphorylase set forth in SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.