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US 12,480,101 B2Granted
Modified host cells for high efficiency production of vanillin
AssigneeAmyris, Inc.
InventorsLauren Raetz, Chad Hansen
Life of this patent
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Abstract
Provided herein are genetically modified host cells, compositions, and methods for improved production of vanillin and/or glucovanillin. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of vanillin and/or glucovanillin and any compound that can be synthesized or biosynthesized from either or both.
Claims15
A genetically modified Saccharomyces cerevisiae host cell that produces vanillin or glucovanillin, the host cell comprising:
- (i) deletion of a homolog of fatty aldehyde dehydrogenase (HFD1) gene;
- and
- (ii) one or more nucleic acids expressing:
- a catechol-O-methyltransferase (COMT);
- an aromatic carboxylic acid reductase (ACAR) from Nocardia iowensis;
- a phosphopantetheinyl transferase (PPTASE);
- and
- a Podospora pauciseta 3-dehydroshikimate dehydratase (AroZ),
- wherein the host cell does not express an Hfd1 protein.
6 more elements
Dependencies are inferred from the claim text.