3D multi-organ co-culture chip

A 3D multi-organ co-culture chip, comprising a chip body, a plurality of groups of culture modules being arranged on the chip body;

• the plurality of groups of culture modules are arranged according to spacing and high flux of porous plates;
• wherein each group of the culture modules comprises:
• a fluid storage hole of which an end is open and is positioned at an upper surface of the chip body;
• a first culture micropore which is positioned below a corresponding fluid storage hole and communicates with the corresponding fluid storage hole;
• the first culture micropore is used for culturing a first organ cell;
• a second culture micropore which is positioned below a corresponding first culture micropore and communicates with the corresponding first culture micropore;
• the second culture micropore is used for culturing a second organ cell;
• a cross section of the fluid storage hole is larger than a cross section of the first culture micropore, and a cross section of the second culture micropore is larger than or equal to the cross section of the first culture micropore;
• a plurality of first fluid operation holes, an end of each of the plurality of the first fluid operation holes is open and is located on the upper surface of the chip body, and another end of the each of the plurality of the first fluid operation holes communicates with the fluid storage holes through first communication channels;
• a plurality of second fluid operation holes, wherein an end of a second fluid operation hole is open and is positioned on the upper surface of the chip body, and another end of the same second fluid operation hole communicates with a corresponding second culture micropore through a channel;
• the plurality of the second fluid operation holes are arranged in pairs;
• each pair of the second fluid operation holes communicates through a through channel;
• and the through channel comprises a first side through channel and a second side through channel, and the first side through channel and the second side through channel are oppositely arranged on two opposite sides of the corresponding second culture micropore;
• a plurality of edge side fluid operating holes, and an end of an edge side fluid operating hole is open and is positioned on the upper surface of the chip body;
• and
• bypass channels, each bypass channel having two ends and a middle part, wherein each end respectively communicates with one of the edge side fluid operating holes, and the middle part communicates with the corresponding second culture micropore;
• a sidewall of the middle part at a communication portion with the second culture micropore is provided with a plurality of micro-channels, wherein cells within the each bypass channel are physically separated from cells within the second culture micropore;
• and bypass channels are used for culturing organ cells which are other types of cells belonging to the same organ as cells in the second culture micropores.