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US 12,479,918 B2Granted
Plasmid constructs for treating cancer and methods of use
AssigneeGrand Decade Developments Limited
InventorsChristopher Twitty, Anandaroop Mukhopadhyay, David A. Canton, Mia Han, Erica Browning
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Abstract
Described are expression cassettes encoding CD3-half-BiTE, CXCL9 and CTLA-4 scFv. The described expression cassettes can be used to treat cancer in a subject. Methods of delivering the expression cassettes to a tumor by direct intratumoral injection and electroporation are also described.
Claims14
An expression cassette comprising the formula represented by:
- P-B-T-B′-T-A
- wherein P is a promoter, A encodes a CXCL9, T is a translation modification element, B encodes IL-12 p35, and B′ encodes IL-12 p40;
- wherein T comprises a 2A peptide selected from the group consisting of:
- a P2A peptide, a T2A peptide, a E2A peptide, and a F2A peptide;
- and
- wherein A encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 58;
- B encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 53;
- and B′ encodes a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 56.
8 more elements
Dependencies are inferred from the claim text.