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US 12,479,878 B2Granted
Crystallization of 2′-FL
AssigneeGlycom A/S
InventorsGetachew S. Molla, Pierre Chassagne
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Abstract
The invention relates to a method for selective crystallization of 2′-FL from an aqueous solution comprising 2′-FL and one or more other fucosylated carbohydrates by adding acetic acid to the solution.
Claims20
A method for crystallizing 2′-FL comprising:
- i) providing an aqueous solution or syrup comprising 2′-FL at a temperature of 35-60° C.,
- ii) while maintaining the temperature of the aqueous solution or syrup at 35-60° C., adding a 10-30 wt % suspension of crystalline 2′-FL in acetic acid to the aqueous solution or syrup comprising 2′-FL, thereby generating a slurry wherein the amount of acetic acid in the slurry, after addition of the suspension of crystalline 2′-FL in acetic acid is 1.5-4 wt %,
- iii) at a temperature of 35-60° C., adding acetic acid to the slurry until the ratio of acetic acid to water is 4-6 to form a diluted slurry,
- iv) obtaining a crystalline mass from the diluted slurry, and
- v) filtering the crystalline 2′-FL from the crystalline mass.
2 more elements
Dependencies are inferred from the claim text.