Preparative chromatography system and method for chromatography separations
AssigneeCytiva BioProcess R&D AB
InventorsMatthew Townsend, Ian Scanlon, Ola Lind, Gunnar Malmquist
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Abstract
The present invention relates to a preparative chromatography system (200, 500, 800) and a chromatography process (400, 700) adapted to repetitive cycling of chromatography volumes. The system (200, 500, 800) comprises at least two upstream pumps (203a, 803a, 203b, 803b) and separate flow paths (220) from process liquid sources to the chromatography device (200, 500, 800). The system (200, 500, 800) is arranged to prime one flow path (220) with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system (200, 500, 800).
Claims22
A method for performing preparative chromatography operating a preparative chromatography system, wherein the preparative chromatography system is configured to operate with at least a first process liquid and a second process liquid, and wherein the preparative chromatography system comprises:
- at least one chromatography device,
- a first process liquid source,
- a second process liquid source,
- a first upper flow path from the first process liquid source to the chromatography device,
- a second upper flow path from the second process liquid source to the chromatography device,
- a first pump in the first upper flow path configured to provide the first process liquid to the chromatography device, and
- a second pump in the second upper flow path configured to provide the second process liquid to the chromatography device,
- wherein the first upper flow path is joined with the second upper flow path at a mixing point, the mixing point being downstream of the first and second pump and upstream of the chromatography device, and
- wherein the method comprises:
- utilizing the first pump to prime the first upper flow path with the first process liquid, and after the priming, utilizing the first pump to provide the first process liquid to the chromatography device;
- and
- utilizing the second pump to prime the second upper flow path with the second process liquid, and after the priming, utilizing the second pump to provide the second process liquid to the chromatography device;
- wherein the chromatography device is a membrane chromatography device.
The method according to any preceding claim, wherein the method for performing preparative comprises an active preparative phase comprising:
- equilibrating or washing the first chromatography device with wash liquid from the wash source via the first upper wash flow path by engaging the wash pump, and during which step at least one of the following steps are performed:
- filling the second chromatography device with feed liquid transferred from the feed source via the second upper feed flow path by the feed pump;
- performing elution in the third chromatography device by transferring eluent liquid from the eluent source via the third upper eluent flow path by the eluent pump, and wherein the eluate is directed to a waste container or to a fraction collector, depending on the content of eluate detected by the monitoring unit;
- filling the third chromatography device with feed liquid transferred from the feed source via the third upper feed flow path by the feed pump;
- performing elution in the second chromatography device by transferring eluent liquid from the eluent source via the second upper eluent flow path by the eluent pump, wherein the eluate is directed to a waste container or to the fraction collector, depending on the content of eluate detected by the monitoring unit, and
- equilibrating or washing the second chromatography device with wash liquid transferred from the wash source via the second upper wash flow path by the wash pump,
- during which at least one of the following steps are performed:
- filling the first chromatography device with feed liquid transferred from the feed source via the first upper feed flow path by the feed pump;
- performing elution in the third chromatography device by transferring eluent liquid from the eluent source via the third upper eluent flow path by the eluent pump, wherein the eluate is directed to a waste container or to the fraction collector, depending on the content of eluate detected by the monitoring unit,
- filling the third chromatography device with feed liquid transferred from the feed source via the third upper feed flow path by the feed pump;
- performing elution in the first chromatography device by transferring eluent liquid from the eluent source via the first upper eluent flow path by the eluent pump, wherein the eluate is directed to a waste container or to the fraction collector, depending on the content of eluate detected by the monitoring unit, and
- equilibrating or washing the third chromatography device with wash liquid transferred from the wash source via the third upper wash flow path by the wash pump,
- and during which step at least one of the following steps are performed:
- filling the first chromatography device with feed liquid transferred from the feed source via the first upper feed flow path by the feed pump;
- performing elution in the second chromatography device by transferring eluent liquid from the eluent source via the second upper eluent flow path by the eluent pump, wherein the eluate is directed to a waste container or to the fraction collector, depending on the content of eluate detected by the monitoring unit,
- filling the second chromatography device with feed liquid transferred from the feed source via the second upper feed flow path by the feed pump;
- performing elution in the first chromatography device by transferring eluent liquid from the eluent source via the first upper eluent flow path by the eluent pump, wherein the eluate is directed to a waste container or to the fraction collector, depending on the content of eluate detected by the monitoring unit.
A preparative chromatography system configured to operate with at least a first process liquid and a second process liquid, the preparative chromatography system comprising at least one chromatography device, a first process liquid source, a second process liquid source, a first upper flow path from the first process liquid source to the chromatography device, a second upper flow path from the second process liquid source to the chromatography device, a first pump positioned in the first upper flow path and arranged to transfer the first process liquid to the chromatography device, and a second pump positioned in the second upper flow path and arranged to transfer the second process liquid to the chromatography device, wherein the first upper flow path is joined with the second upper flow path at a mixing point, the mixing point being downstream of the first and second pump and upstream of the chromatography device, characterized in that the system is configured to
- prime the first upper flow path with the first process liquid via the first pump, and after the priming, utilizing the first pump to transfer the first process liquid to the chromatography device;
- and
- prime the second upper flow path with the second process liquid, via the second pump, and after the priming, utilizing the second pump to transfer the second process liquid to the chromatography device;
- wherein the chromatography device is a membrane chromatography device.
Dependencies are inferred from the claim text.