Method for manufacturing cell population including liver precursor cells
AssigneeNagasaki University
InventorsYusuke Sakai, Susumu Eguchi
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Abstract
A method for producing a cell population containing liver progenitor cells, including the steps of (1) preparing a culture substratum containing a cell population comprising liver progenitor cells and fibroblasts, (2) physically removing the fibroblast colony from the culture substratum, (3) detaching cells from the culture substratum, and recovering the detached cells, and (4) culturing the cells recovered in the step (3) on a collagen-coated culture substratum, and recovering the cells not adhered to the culture substratum is provided by the present invention.
Claims7
A method for producing a cell population comprising liver progenitor cells wherein purity of liver progenitor cells is higher than that in a starting cell population comprising liver progenitor cells and fibroblasts, comprising the steps of
- (1) preparing a culture substratum comprising the starting cell population comprising liver progenitor cells and fibroblasts,
- (2) physically removing the fibroblast colony from the culture substratum,
- (3) detaching cells from the culture substratum, and recovering the detached cells, and
- (3′) culturing the recovered cells on a gelatin-coated culture substratum, and recovering the cells not adhered to the culture substratum,
- (4) culturing the cells recovered in the step (3) on a collagen-coated culture substratum, and recovering the cells not adhered to the culture substratum, and
- (5) culturing the recovered cells on a collagen-coated culture substratum, and removing the cells not adhered to the culture substratum
- thereby obtaining a cell population comprising liver progenitor cells having a reduced number of fibroblasts compared to the starting cell population.
Dependencies are inferred from the claim text.