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US 12,476,009 B2Granted
Immune age and use thereof
AssigneeTechnion Research & Development Foundation Limited
InventorsShai S. Shen-Orr, Yishai Menachem Pickman, Ayelet Alpert
Life of this patent
Estimating the term…
Abstract
Methods of determining an immunological age of a subject comprising measuring relative abundance of at least 3 immune cell populations in a blood sample from the subject and determining an immunological age of the subject based on the measured relative abundances are provided. Methods of diagnosing increased risk of illness and mortality are also provided, as are kits for performing these methods.
Claims11
A method of diagnosing and treating an increased risk of cardiovascular disease of a subject, comprising:
- (a) measuring relative abundance of at least 3 immune cell populations in a blood sample from said subject;
- (b) determining an immunological age of said subject by at least one of:
- (i) comparing said measurement of relative abundance of immune cell populations to a dataset of measurements of immune cell population relative abundance in subjects with predetermined immunological ages, wherein said comparing comprises employing a distance metric with respect to immune cell population abundance in said sample and samples of said dataset or selecting from said dataset individuals with a smallest distance with respect to relative abundance of said at least 3 immune cell populations and averaging an immunological age of said selected individuals;
- and
- (ii) combining said measurement of relative abundances with data of measurements of relative abundance from at least 19 other subjects to produce a database, calculating from said database a trajectory for all at least 20 subjects based on said measurements of relative abundance, wherein said calculating a trajectory comprises applying a diffusion-pseudotime algorithm to said population relative abundancies;
- (c) diagnosing said subject whose immunological age is greater than said subject's chronological age with an increased risk of cardiovascular disease or diagnosing said subject whose immunological age is greater than an immunological age of at least one other subject of the same chronological age with an increased relative risk of cardiovascular disease;
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- (d) administering to said subject diagnosed with increased risk of cardiovascular disease or relative increased risk of cardiovascular disease a cardiovascular medication selected from:
- a statin, aspirin, an anticoagulant, an antiplatelet agent, an angiotensin receptor blocker, a beta blocker, a calcium channel blocker, a diuretic and a vasodilator;
- thereby diagnosing and treating an increased risk of cardiovascular disease in a subject.
7 more elements
Dependencies are inferred from the claim text.