Pulse stable tracer methods for detection of short-chain fatty acids
OwnerNicolaas E. Deutz, Marielle P. Engelen +2inventors
InventorsNicolaas E. Deutz, Marielle P. Engelen, Gabrielle A. Ten Have, John J. Thaden
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Abstract
Provided herein are methods for determining a metabolic rate for at least one endogenous short-chain fatty acid in a subject, for diagnosing a pulmonary disease in a subject and for diagnosing a neurological disorder in a subject. Generally in the methods stable isotope labeled short-chain fatty acid are pulsed and metabolic rates are calculated in a first baseline blood sample and in a series of second blood samples obtained at intervals. The metabolic rates provide information about fatty acid metabolism and production.
Claims4
A method for diagnosing a deficiency in short-chain fatty acid metabolism in a subject, comprising the steps of:
- a) drawing a baseline first blood sample from a fasting subject;
- b) administering to the subject:
- at least one of 13C-acetate short-chain fatty acid, 13C-propionate short-chain fatty acid, 13C-butyrate short-chain fatty acid, 13C-iso-butyrate short-chain fatty acid, 13C-valerate short-chain fatty acid, or 13C-iso-valerate short-chain fatty acid via intravenous pulsing;
- c) drawing a series of second arterialized blood samples at intervals from the subject;
- d) performing a compartmental modeling analysis utilizing R-language algorithms on the baseline first blood sample and on each of the series of second arterialized blood samples to determine concentrations of the 13C-isotopes from the at least one short-chain fatty acid;
- e) administering orally a soluble fiber to the subject;
- f) drawing another baseline blood sample;
- g) drawing another series of arterialized blood samples at intervals from the subject;
- and
- h) performing another compartmental modeling analysis on each of the arterialized blood samples drawn after administering the soluble fiber to determine concentrations of the 13C-isotopes from the at least one short-chain fatty acid;
- wherein a concentration of the 13C-isotopes from the at least one short-chain fatty acid in the presence of the soluble fiber substantially equal to the concentration of the 13C-isotopes from the at least one short-chain fatty acid without the presence of the soluble fiber indicates that short-chain fatty acid production in the subject is deficient.
Dependencies are inferred from the claim text.