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US 12,474,345 B2Granted
Chemigenetic voltage indicators
AssigneeHoward Hughes Medical Institute
InventorsEric R. Schreiter, Luke D. Lavis, Ahmed Abdelfattah
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Abstract
Provided herein are a voltage indicator and a method of measuring voltage. The voltage indicator includes a membrane-localized voltage-sensitive protein coupled to a capture protein. The method of measuring voltage includes administering a voltage indicator including a membrane-localized voltage-sensitive protein coupled to a capture protein, and determining changes in fluorescence of a small-molecule fluorescent dye captured by the capture protein.
Claims20
A voltage indicator comprising:
- a polypeptide comprising a membrane-localized voltage-sensitive protein and a capture protein, and
- a cell-permeable fluorescent dye, wherein the voltage-sensitive protein is coupled to the capture protein and the capture protein is covalently or noncovalently labeled with the cell-permeable fluorescent dye.
A method of measuring voltage, the method comprising
- administering
- (a) a polypeptide comprising a membrane-localized voltage-sensitive protein coupled to a capture protein, and
- (b) a cell-permeable fluorescent dye, wherein the capture protein is capable of capturing the cell-permeable fluorescent dye;
- and
- measuring changes in fluorescence when the cell-permeable fluorescent dye is captured by the capture protein of the polypeptide.
2 more elements
Dependencies are inferred from the claim text.