Spectral imaging platform for infectious disease diagnosis

A method of diagnosing an infectious disease using a spectral imaging platform, comprising:

• (a) having a sample with an added fluorescent dye;
• (b) triggering a spectral shift on an order of 10 nm by exciting the sample with added fluorescent dye with a laser excitation of about 405 nm;
• (b) imaging the spectral shift using an imaging module with a magnification ranging of about 10×;
• (c) long pass filtering the spectral shifted images using a long pass filter with a cutoff wavelength of about 435 nm;
• (d) collecting the long-passed-filtered spectral shifted images using a CMOS sensor having a color filter array in Bayer arrangements for collecting light in a plurality of channels;
• (e) analyzing color pixels of the obtained images through the CMOS sensor to determine a spectral shift using color pixel signal ratios using an automated computational module;
• and
• (f) classifying and diagnosing an infectious disease from the sample using the output from the automated computational module.