Method for establishing fingerprint of traditional chinese medicine compound or its preparation with improving cognition

A method for establishing fingerprint of traditional Chinese medicine compound or its preparation with improving cognition, wherein the traditional Chinese medicine compound is made from following raw materials:

• 1-20 parts by weight of Gastrodiae Rhizoma, 1-15 parts by weight of Polygala tenuifolia, 1-30 parts by weight of Acorus tatarinowii, 0.1-10 parts by weight of Cistanche deserticola Ma, 0.1-10 parts by weight of Rehmanniae Radix Praeparata, and 0.01-1 parts by weight of curcumin;
• a chromatogram of a test sample solution is established by high performance liquid chromatography, chromatographic conditions are as follows:
• a chromatographic column is octadecyl silane bonded silica gel chromatographic column;
• a column temperature is 25˜35° C.;
• a flow rate is 0.9˜1.1 ml/min;
• detection wavelengths are 210˜230 nm and 310˜330 nm respectively;
• a mobile phase A is acetonitrile and a mobile phase B is water;
• and a gradient elution procedure is as follows:
• Time/min
• Mobile phase A/%
• Mobile phase B/%
• 0.00~5.00
• 5
• 95
• 5.00~8.00
• 5→8
• 95→92
• 8.00~11.00
• 8
• 92
• 11.00~17.00
• 8→13
• 92→87
• 17.00~23.00
• 13
• 87
• 23.00~26.00
• 13→17
• 87→83
• 26.00~30.00
• 17
• 83
• 30.00~31.00
• 17→21.5
• 83→78.5
• 31.00~73.00
• 21.5→31
• 78.5→69
• 73.00~93.00
• 31→50
• 69→50
• 93.00~103.00
• 50→60
• 50→40
• 103.00~108
• 60→70
• 40→30
• 108.00~110.00
• 70→90
• 30→10
• 110.00~120.00
• 90
• 10
• wherein a preparation method of the test sample solution is as follows:
• taking the traditional Chinese medicine compound or its preparation, mixing it with an acetonitrile aqueous solution, wherein a ratio of the traditional Chinese medicine compound or its preparation to the acetonitrile aqueous solution is (0.5˜1.5):50 in g/ml;
• performing an ultrasonic treatment;
• filtering and taking a subsequent filtrate.