Kit and method for monitoring oral inflammation associated with periodontal diseases

A method for detection of a biomarker indicative of an inflammation in a user's oral cavity, the method comprising

• pre-rinsing the oral cavity including introducing an oral pre-rinse substance in the oral cavity, then disposing of the pre-rinse substance from the oral cavity;
• introducing, after delaying for between about 110 to about 130 seconds after disposing of the pre-rinse substance from the oral cavity, a predetermined amount of a rinsing substance in the oral cavity, the rinsing substance being water of selected quality standard;
• rinsing the user's oral cavity for a fixed duration with the predetermined amount of the rinsing substance;
• collecting the biomarker released in the rinsing substance from the oral cavity from the rinsing of the user's oral cavity by retrieving a sample volume of the rinsing substance in a receptacle;
• immersing a colourimetric indicator located on a test strip into the sample volume to absorb, into the colourimetric indicator, an amount of the biomarker, the colourimetric indicator reactive to the biomarker;
• removing the test strip from the sample volume after a given time period after immersing it, thereby allowing a reaction of the colourimetric indicator to the biomarker;
• and
• after a predetermined duration following the removing of the colourimetric indicator of the test strip from the sample volume, identifying a level of inflammation of the oral cavity by comparing a colour of the reacted colourimetric indicator of the test strip to a plurality of values of a colourimetric chart to detect an occurrence of inflammation in the user's oral cavity based on a matching of the colour with one of the plurality of values of the colourimetric chart.

A method for detection of a biomarker indicative of an inflammation in a user's oral cavity, the method comprising:

• pre-rinsing the oral cavity including introducing an oral pre-rinse substance in the oral cavity, then disposing of the pre-rinse substance from the oral cavity;
• introducing, after delaying for between about 110 to about 130 seconds after disposing of the pre-rinse substance from the oral cavity, a predetermined amount of a rinsing substance of a dose container in the oral cavity, the rinsing substance being water of selected quality standard;
• rinsing the user's oral cavity for about 25 to 35 seconds with the predetermined amount of the rinsing substance;
• collecting the biomarker released in the rinsing substance from the oral cavity from the rinsing of the user's oral cavity by retrieving a sample volume of about 10 mL of the rinsing substance from the rinsing of the oral cavity in a receptacle;
• immersing a colourimetric indicator located on a test strip into the sample volume to absorb, into the colourimetric indicator, an amount of the biomarker, the colourimetric indicator reactive to the biomarker;
• removing the test strip from the sample volume after a given time period after immersing it, thereby allowing a reaction of the colourimetric indicator to the biomarker;
• and
• after between about 50 to 70 seconds following the removing of the colourimetric indicator of the test strip from the sample volume, identifying a level of inflammation of the oral cavity by comparing a colour of the reacted colourimetric indicator of the test strip to a plurality of values of a colourimetric chart to detect an occurrence of inflammation in the user's oral cavity based on a matching of the colour with one of the plurality of values of the colourimetric chart.