Method for evaluation of viability of viruses with lymphotropism properties
AssigneeOvik Leonardovich Mkrtchyan
InventorNariman Gulyamov
Life of this patent
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Abstract
Methods and techniques to increase the reliability of detecting virus infections, particularly lymphotropism, to eliminate false negative reactions in testing blood for the presence of lymphotropic viruses during enzyme immunoassay (EIA) and polymerase chain reaction (PCR) testing, and to better detect viruses with lymphotropism in biological materials having a concentration of virus particles lower than the sensitivity threshold of existing EIA and PCR methods, thereby making the techniques of the present invention more reliable.
Claims22
A method for the viability detection of viruses with lymphotropism, comprising:
- collecting a biological material from a patient, wherein said biological material has a low virus concentration therein;
- forming an admixture of an equal amount of said biological material from said patient with a lymphocyte suspension from a healthy human subject;
- stirring and incubating the admixture;
- washing-out the lymphocytes in the admixture;
- concentrating lymphocyte cytoplasm content within said admixture by removing lymphocyte membranes from the admixture;
- and
- detecting, within said lymphocyte cytoplasm content, the presence or absence of the virus RNA or DNA,
- wherein the presence of the virus RNA or DNA in the lymphocyte cytoplasm content, with an earlier non-detection of infection, indicates the preserved viability of viruses in said biological sample of said patient,
- wherein the absence of the virus RNA or in the lymphocyte cytoplasm content indicates the loss of virus viability in said biological sample of said patient.
A method for the viability detection of viruses with lymphotropism properties, comprising:
- collecting a biological material from a patient, wherein said biological material has a low virus concentration;
- collecting a lymphocyte suspension from a blood sample of at least one healthy human subject;
- forming an admixture of an equal amount of said biological material with said lymphocyte suspension;
- stirring and incubating the admixture;
- washing-out the lymphocytes from the admixture;
- concentrating lymphocyte cytoplasm content by removing lymphocyte membranes from the admixture, separating the lymphocyte cytoplasm content by centrifugation;
- and
- detecting, within the lymphocyte cytoplasm content, the presence or absence of the virus RNA or DNA,
- wherein the presence of the virus RNA or DNA in the lymphocyte cytoplasm content, with an earlier non-detection of infection, indicates the preserved viability of viruses in said biological sample of said patient,
- wherein the absence of the virus RNA or in the lymphocyte cytoplasm content indicates the loss of virus viability in said biological sample of said patient.
Dependencies are inferred from the claim text.