Method and agents for detecting luciferase activity
AssigneeLight Bio, Inc.
InventorsIl'ya Viktorovich Yampol'skiy, Valentin Nikolaevich Petushkov, Konstantin Viktorovich Purtov, Natal'ya Sergeevna Rodionova, Mikhail Sergeevich Baranov
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Abstract
The invention provides methods and reagents for detecting luciferase in biological samples. The methods and reagents of the present invention allow detecting fungal luciferase or a functional analog thereof.
Claims11
A biological sample comprising at least one cell containing at least one of 3-hydroxyhispidin and 3-hydroxybisnoryangonin, wherein the biological sample is not obtained from a higher fungus.
A method of producing 3-hydroxyhispidin or 3-hydroxybisnoryangonin in a biological sample, the method comprising:
- combining, in the biological sample, at least one of hispidin and bisnoryangonin, with at least one of NADPH and NADH, and an isolated hispidin 3-hydroxylase.
A method for detecting bioluminescence in a biological sample having at least one cell, the method comprising:
- providing an isolated hispidin 3-hydroxylase to the biological sample;
- providing an isolated fungal luciferase to the biological sample;
- providing one of hispidin and bisnoryangonin, and NAD(P)H to the biological sample;
- and
- detecting bioluminescence.
Dependencies are inferred from the claim text.