Carbonyl reductase, nucleic acid encoding same, and method for producing optically active compound using same

A method for producing an optically active compound represented by the following formula (IV):

• wherein:
• R is a hydrogen atom, an alkyl group, or an aryl group, and
• is a substituent having an aromatic ring or a heterocycle, the method comprising:
• asymmetrically reducing a carbonyl group-containing compound selected from the group consisting of compounds represented by the following formulas (I), (II) and (III):
• in the above-mentioned formulas (I), (II) and (III), R is as defined above, and
• is as defined above, by bringing the carbonyl group-containing compound into contact with an enzyme, a microorganism or cell having the ability to produce the enzyme, a processed product of the microorganism or cell, or a culture medium containing the enzyme obtained by culturing the microorganism or cell:
• wherein the enzyme is a carbonyl reductase variant comprising an amino acid sequence having at least 90% and less than 100% sequence identity to SEQ ID NO:
• 1 and having carbonyl reductase activity, the variant comprising at least one or more mutation selected from the group consisting of:
• (a) a mutation in which the 54th aspartic acid in SEQ ID NO:
• 1 is substituted by valine;
• (b) a mutation in which the 157th methionine in SEQ ID NO:
• 1 is substituted by valine;
• (c) a mutation in which the 170th alanine in SEQ ID NO:
• 1 is substituted by serine;
• (d) a mutation in which the 211th isoleucine in SEQ ID NO:
• 1 is substituted by alanine or asparagine;
• (e) a mutation in which the 214th methionine in SEQ ID NO:
• 1 is substituted by leucine;
• and
• (f) a mutation in which the 249th methionine in SEQ ID NO:
• 1 is substituted by leucine.