Modification of small RNAs for therapeutic uses
AssigneeCity of Hope
InventorsJohn J. Rossi, Minsun Song
Life of this patent
Estimating the term…
Abstract
Provided are methods for improving stability of small therapeutic RNAs by adding one or more non-templated nucleotides such as cytidines and uridines to the 3′ end of the small therapeutic RNAs. Also disclosed are modified small therapeutic RNAs comprising one or more non-templated nucleotides such as cytidines and uridines at the 3′ end and pharmaceutical compositions comprising such modified small therapeutic RNAs.
Claims9
A method of improving an in vivo stability level of a Dicer-derived small therapeutic RNA molecule relative to a control, comprising contacting the Dicer-derived small therapeutic RNA molecule with a 3′ to 5′ exonuclease in vitro to remove a 3′ overhang from the Dicer-derived small therapeutic RNA molecule prior to adding a non-templated mono-uridine nucleotide to the 3′ end of the Dicer-derived small therapeutic RNA molecule in vitro.
A method of improving an in vivo stability level of a Dicer-derived small therapeutic RNA molecule relative to a control, comprising contacting the Dicer-derived small therapeutic RNA molecule with a 3′ to 5′ exonuclease in vitro to remove a 3′ overhang from the Dicer-derived small therapeutic RNA molecule prior to adding a non-templated mono-cytidine nucleotide to the 3′ end of the Dicer-derived small therapeutic RNA molecule in vitro.
Dependencies are inferred from the claim text.