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US 12,473,542 B2Granted
β-amylase and method for utilization and production thereof
AssigneeUniversity Public Corporation Osaka
InventorsJun-ichi Sumitani, Shuji Tani, Takashi Kawaguchi
Life of this patent
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Abstract
An object is to obtain a β-amylase having heat resistance even to high temperatures exceeding 60° C. By screening β-amylase producing bacteria from a soil sample, a novel Bacillus halosaccharovorans strain having a novel β-amylase with heat resistance and the β-amylase from the novel B. halosaccharovorans strain were obtained.
Claims20
A vector comprising a nucleic acid sequence encoding, or an enzyme preparation comprising a β-amylase having:
- (i) the amino acid sequence set forth in SEQ ID NO. 2 or 12;
- (ii) the amino acid sequence set forth in SEQ ID NO. 2 or 12 except for one to nine amino acid deletions, substitutions, insertions and/or additions;
- (iii) an amino acid sequence having 90% or more sequence identity to SEQ ID NO. 2 or 12;
- (iv) the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25;
- (v) the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25 except for one to nine amino acid deletions, substitutions, insertions and/or additions;
- or
- (vi) an amino acid sequence having 90% or more sequence identity to the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25.
4 more elements
A method of producing maltose, the method comprising a step of treating a starch with
- (i) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in SEQ ID NO. 2 or 12;
- (ii) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in SEQ ID NO. 2 or 12 except for one to nine amino acid deletions, substitutions, insertions and/or additions;
- (iii) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence having 90% or more sequence identity to SEQ ID NO. 2 or 12;
- (iv) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25;
- (v) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25 except for one to nine amino acid deletions, substitutions, insertions and/or additions;
- (vi) a β-amylase or enzyme preparation comprising a β-amylase having an amino acid sequence having 90% or more sequence identity to the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25;
- or
- (vii) a vector or microorganism comprising a nucleic acid molecule encoding the β-amylase of one of (i)-(vi).
5 more elements
A method of modifying a food product, the method comprising a step of treating the food product with
- (i) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in SEQ ID NO. 2 or 12;
- (ii) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in SEQ ID NO. 2 or 12 except for one to nine amino acid deletions, substitutions, insertions and/or additions;
- (iii) a β-amylase or enzyme preparation comprising a β-amylase having an amino acid sequence having 90% or more sequence identity to SEQ ID NO. 2 or 12;
- (iv) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25;
- (v) a β-amylase or enzyme preparation comprising a β-amylase having the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25 except for one to nine amino acid deletions, substitutions, insertions and/or additions;
- (vi) a β-amylase or enzyme preparation comprising a β-amylase having an amino acid sequence having 90% or more sequence identity to the amino acid sequence set forth in any one of SEQ ID NOS. 18 to 25;
- or
- (vii) a vector or microorganism comprising a nucleic acid molecule encoding the β-amylase of one of (i)-(vi).
5 more elements
Dependencies are inferred from the claim text.