Escherichia coli for synthesizing l-valine, construction method and use thereof

A recombinant Escherichia coli for synthesizing L-valine, wherein the recombinant Escherichia coli is an Escherichia coli for synthesizing L-valine designated as Escherichia coli W3110 as deposited in China Center for Type Culture Collection (Address:

• Bavi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 2022293 on Mar. 18, 2022, and comprising further modifications as follows:
• dicarboxylic acid reductoisomerase gene ilvC, dihydroxy acid dehydratase gene ilvD and branched chain amino acid aminotransferase gene ilvE are overexpressed;
• branched-chain amino acid transporter gene brnQ is knocked out, and branched-chain amino acid output protein gene brnFE is integrated into the site of the knocked-out branched-chain amino acid transporter gene brnQ;
• phosphogluconate dehydratase gene edd and KHG/KDPG aldolase gene eda are integrated;
• and a transcription regulation factor is overexpressed to obtain the recombinant Escherichia coli,
• wherein the transcription regulation factor is a positive transcription regulation factor and/or a negative transcription regulation factor;
• the positive transcription regulation factor is selected form the group consisting of a DNA binding transcription double regulator pdhR, a DNA binding transcription double regulator crp and a DNA binding transcription double regulator lrp;
• and the negative transcription regulation factor is RNA polymerase sigma factor rpoS, and
• wherein the transcription regulation factor is overexpressed from a ptrc99A or ptrc28A vector.

A method for constructing a recombinant Escherichia coli for synthesizing L-valine, comprising:

• providing an Escherichia coli for synthesizing L-valine designated as Escherichia coli W3110 as deposited in China Center for Type Culture Collection (Address:
• Bavi Road, Wuchang District, Wuhan City, Hubei Province) under the Accession No. CCTCC M 2022293 on Mar. 18, 2022, and in the Escherichia coli for synthesizing L-valine further introducing the following:
• overexpressing a transcription regulation factor, wherein the transcription regulation factor is a positive transcription regulation factor and/or a negative transcription regulation factor;
• the positive transcription regulation factor is selected from the group consisting of a DNA binding transcription double regulator pdhR, a DNA binding transcription double regulator crp and a DNA binding transcription double regulator Irp;
• and the negative transcription regulation factor is RNA polymerase sigma factor rpoS;
• and/or overexpressing dicarboxylic acid reductoisomerase gene ilvC, dihydroxy acid dehydratase gene ilvD and branched chain amino acid aminotransferase gene ilvE;
• and/or knocking out branched-chain amino acid transporter gene brnQ, integrating branched-chain amino acid output protein gene brnFE into the site of the knocked-out branched-chain amino acid transporter gene brnQ, and making double copies of the brnFE gene;
• and/or integrating phosphogluconate dehydratase gene edd and KHG/KDPG aldolase gene eda to obtain the recombinant Escherichia coli.