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US 12,472,499 B2Granted
Particle separator system, materials, and methods of use
AssigneeLevitasbio, Inc.
InventorsGeoffrey Facer, Theodorus Evan de Groot, Kevin Travers, Lesley Suzanne Weaver
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Abstract
The present invention relates generally to systems, materials, and methods utilizing magnetic levitation to affect separation and/or concentration of particulate containing samples, such as cells or biomolecules, within a medium. In some embodiments, the methods are used for the separation of a mixture of live cells and dead cells, and to isolate particle depleted medium.
Claims25
A method for separation of a mixture of live cells and dead cells comprising:
- providing a flowcell cartridge comprising a processing channel, and a plurality of outlet channels, wherein said processing channel has a top vertical surface and a bottom vertical surface;
- flowing a sample solution comprising live cells and dead cells and a paramagnetic compound into the processing channel;
- placing the flowcell cartridge in a magnetic field substantially aligned parallel to the processing channel;
- maintaining the processing channel and the sample solution contained therein entirely within the magnetic field in a stopped flow condition for a period of time sufficient to separate live cells and dead cells by a vertical distance within the processing channel;
- and
- simultaneously withdrawing a sample fraction enriched with live cells and a sample fraction enriched with dead cells into the outlet channels;
- wherein the paramagnetic compound is dissolved in solution and is present in an amount of from about 50 mM to about 200 mM.
4 more elements
Dependencies are inferred from the claim text.