Multiplexed bead-based analytical assays
AssigneeAdeptrix Corp.
InventorVladislav B. Bergo
Life of this patent
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Abstract
Methods for performing bead-based analytical assays for detecting changes in abundance of target analytes in biological samples are disclosed. In an embodiment, a method involves eluting bead-captured analytes from a bead array under conditions that preserve spatial localization of the eluted analytes.
Claims9
A method for eluting an analyte from a bead array, the bead array comprising a bead positioned in a microwell, the bead being associated with an analyte, the microwell containing an aqueous medium, the method comprising steps of:
- depositing an elution medium into the microwell, the elution medium comprising a dissolved matrix for mass spectrometry (MS) and a solvent, wherein a rate, at which the elution medium is being deposited into the microwell, is approximately equivalent to a rate, at which the solvent is escaping from the microwell via evaporation, such that (i) the bead is continuously exposed to the solvent, (ii) a mixture that forms in the microwell has a higher concentration of the dissolved matrix relative to the elution medium and (iii) the analyte is eluted from the bead, and wherein the elution medium is being deposited using a programmable device capable of producing an aerosol,
- supplying at least one matrix deposition parameter value to the programmable device, the at least one matrix deposition parameter value being selected according to an ambient air humidity level,
- providing an amount of time for the eluted analyte to remain in the mixture and mix with the dissolved matrix, and
- allowing the solvent to evaporate, thereby causing the analyte and the matrix to co-crystallize.
A method for eluting an analyte from a bead array, the bead array comprising a bead positioned in a microwell, the bead being associated with an analyte, the microwell containing an aqueous medium, the method comprising steps of:
- depositing an elution medium into the microwell, the elution medium comprising a dissolved matrix for mass spectrometry (MS) and a solvent, wherein a rate, at which the elution medium is being deposited into the microwell, is approximately equivalent to a rate, at which the solvent is escaping from the microwell via evaporation, such that a mixture of the aqueous medium and the elution medium is formed inside the microwell under conditions that substantially prevent the matrix from precipitating from the mixture,
- causing the analyte to elute from the bead, wherein the analyte elution occurs between 5 and 30 minutes before the matrix precipitation, and
- allowing the solvent to escape the microwell via evaporation, thereby causing the matrix to precipitate.
A method for eluting an analyte from a bead array, the bead array comprising a bead positioned in a microwell, the bead being associated with an analyte, the microwell containing an aqueous medium, the method comprising steps of:
- depositing an elution medium into the microwell, the elution medium comprising a dissolved matrix for mass spectrometry (MS) and a solvent, wherein a rate, at which the elution medium is being deposited into the microwell, is approximately equivalent to a rate, at which the solvent is escaping from the microwell via evaporation, such that a concentration of the matrix in the mixture exceeds a concentration of the matrix in the elution medium,
- causing the analyte to elute from the bead and remain in the mixture for between 5 and 30 minutes, and
- allowing the solvent to escape the microwell via evaporation, thereby causing the dissolved matrix to co-crystallize with the eluted analyte.
Dependencies are inferred from the claim text.