Immunochromatographic device for extracting and measuring sugar chain antigen

An immunochromatographic device comprising an immunochromatographic test strip for extracting and measuring a sugar chain antigen in a specimen and a cassette for accommodating the test strip, wherein:

• the immunochromatographic test strip comprises a sample pad to which a specimen mixed with the nitrite or acid solution is added, a label region comprising a labeled antibody obtained by labeling an antibody against the sugar chain antigen, and a detection region on which the antibody against the sugar chain antigen is immobilized, an antibody-sugar chain antigen-labeled antibody complex is formed in the detection region to measure the sugar chain antigen, and the immunochromatographic test strip has a region impregnated with a neutralizing reagent upstream of the label region and further has, upstream of the region impregnated with the neutralizing reagent, a region impregnated with a solid acid reagent when the specimen mixed with the nitrite is used or a region impregnated with the nitrite when the specimen mixed with the acid solution is used,
• the cassette is composed of an upper lid portion and a lower portion, the upper lid portion comprises an upper support having a protrusion that supports the upper side of the immunochromatographic test strip, and the lower cassette portion comprises a lower support comprising a protrusion that supports the lower side of the immunochromatographic test strip,
• the immunochromatographic device comprises a specimen dropping port on top of a sample pad of the immunochromatographic test strip, and
• the upper support that supports the immunochromatographic test strip has the following features (i) to (iii) and the lower support that support that supports the immunochromatographic test strip has the following features (iv) to (vi):
• (i) the upper support is provided to form an edge of an opening on the side of the dropping port opposite from the side to which the specimen is added, and is formed of a sloped protrusion;
• (ii) when the upper support pushes the upper side of the sample pad of the immunochromatographic test strip in a downward direction, the height of the protrusion becomes smaller toward the downstream side of the immunochromatographic test strip, that is, the protrusion has an increasing slope toward the downstream side of the immunochromatographic test strip;
• (iii) when the upper support of the upper lid portion pushes the sample pad, the pressure becomes lower in a downward direction;
• (iv) the lower support is formed of a sloped protrusion provided in a region corresponding to the specimen dropping port of the lower cassette portion, wherein the region corresponding to the specimen dropping port of the lower cassette portion is a region overlapping with the opening of the specimen dropping port when the lower cassette portion is covered by the upper lid portion;
• (v) when the lower support pushes the sample pad of the immunochromatographic test strip, the height of the protrusion becomes smaller toward the downstream side of the immunochromatographic test strip, that is, the protrusion has a decreasing slope toward the downstream side of the immunochromatographic test strip;
• (vi) the pressure to push the sample pad becomes lower in a downward direction.