Hybridoma cell line secreting an ActA monoclonal antibody and use thereof

A competitive ELISA detection kit for detecting Listeria monocytogenes infection, comprising an Actin assembly-inducing protein (ActA) monoclonal antibody or a polynucleotide;

• wherein the ActA monoclonal antibody comprises a heavy chain and a light chain, wherein the light chain comprises three complementarity determining regions with amino acid sequences shown as SEQ ID NO:1, SEQ ID NO:2, and SEQ ID NO:3, respectively;
• and the heavy chain comprises three complementarity determining regions with amino acid sequences shown as SEQ ID NO:4, SEQ ID NO:5, and SEQ ID NO:6, respectively;
• wherein the polynucleotide encodes the ActA monoclonal antibody, wherein a nucleotide sequence encoding the light chain of the ActA monoclonal antibody is shown as SEQ ID NO:
• 8, and a nucleotide sequence encoding the heavy chain of the ActA monoclonal antibody is shown as SEQ ID NO:
• 10;
• wherein the competitive ELISA detection kit further comprises one or more of the following:
• an enzyme-labeled antibody, a coated antigen, a positive and/or negative control serum for Listeria monocytogenes, a coating buffer, a blocking buffer, a dilution buffer, a washing buffer, a substrate solution, and a reaction stop solution.