Method for producing secreted β-galactosidase
AssigneeKabushiki Kaisha Yakult Honsha
InventorsEiji Ishikawa, Masakazu Ikeda, Minako Anbe, Hiroshi Hatano
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Abstract
A method for producing a secreted β-galactosidase, characterized by integrating a non-secreted β-galactosidase gene derived from a basidiomycetous yeast into Aspergillus oryzae to produce a secreted β-galactosidase, and a method for producing a galactooligosaccharide using a β-galactosidase produced by the method facilitate the production of a galactooligosaccharide.
Claims3
A method for producing a secreted β-galactosidase, comprising integrating a non-secreted β-galactosidase gene derived from Sporobolomyces singularis and a signal sequence into Aspergillus oryzae to produce a secreted β-galactosidase, and
- culturing the Aspergillus oryzae in a CDD medium,
- wherein the non-secreted β-galactosidase gene derived from Sporobolomyces singularis and the signal sequence has a sequence of SEQ ID NO:
- 1, 3 or 5, and
- wherein the CDD medium comprises dextrin, glucose, NH4Cl, KCl, K2HPO4, MgSO4·7H2O, CuSO4·5H2O, FeSO4·7H2O, ZnSO4·7H2O, MnSO4·5H2O, AlCl3, and MOPS-NaOH buffer pH 7.0.
Dependencies are inferred from the claim text.