Cell transfection
OwnerAliah Mohammed Abuammahinventor
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Abstract
A method of preparing a slide for use in the transfection of cells is disclosed. The method comprises the steps of a) providing a biological material composition having a temperature of from 15 to 25° C.; b) providing the slide having a temperature of from 26 to 50° C.; and c) depositing the biological material composition onto a surface of the slide, to at least partially adhere the biological material composition to the slide. The biological material composition material composition may comprise siRNA or gRNA, for example. The method may provide an improved adherence of the biological material to the slide and subsequently facilitate transfection of cells on the slide. A slide produced by the method, a composition for applying to the slide and a method of transfecting a cell are also disclosed.
Claims11
A method of preparing a surface for deposition of cells, the method comprising the steps of:
- a) providing a biological material composition having a temperature of from 15 to 25° C.;
- b) providing the surface having a temperature of from 30 to 50° C.;
- and
- c) depositing the biological material composition having a temperature of from 15 to 25° C. onto the surface having a temperature of from 26 to 50° C., to at least partially adhere the biological material composition to the surface,
- wherein the difference in temperature of the biological material composition and the surface in step c) is at least 10° C.
Dependencies are inferred from the claim text.