Recombinant AAV vectors useful for reducing immunity against transgene products

A method of delivering a transgene to a subject, the method comprising administering to the subject a recombinant Adeno-Associated Virus (rAAV) comprising the transgene, wherein the transgene encodes an RNA transcript that comprises two different immune-associated miRNA binding sites, wherein the two different immune-associated miRNA binding sites bind a miRNA pair selected from:

• (i) miR-142-3p and miR-155, (ii) miR-181a and miR-21, (iii) miR-16-1 and miR-150, (iv) miR-146 and miR-181la, (v) miR-31 and miR-181a, (vi) miR-146a and miR-16-1, (vii) miR-150 and miR-18 1a, (viii) miR-16-1 and miR-181a, and (ix) miR-142-3p and miR-223.