Methods of culturing human pluripotent cells
AssigneeHadasit Medical Research Services and Development Ltd.
InventorsBenjamin Eithan Reubinoff, Michal Gropp
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Abstract
A culture of human pluripotent stem cells (hPSCs) is disclosed. In the culture, more than 50% of the hPSCs are formative hPSCs and are capable of renewing. Uses thereof are also disclosed.
Claims8
A method of enriching for a population of formative hPSCs which express WNT receptors but do not express nuclear B-catenin, the method comprising culturing non-formative hPSCs on an adherent surface which comprises a coating of a single protein, said single protein being laminin, said culturing being effected in a culture medium comprising fibroblast growth factor 2 (FGF2), thereby enriching for the population of formative hPSCs which express WNT receptors but do not express nuclear B-catenin.
Dependencies are inferred from the claim text.