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US 12,467,026 B2Granted
Cell culture tool and method for producing cell culture tool
AssigneeKataoka Corporation
InventorJunichi Matsumoto
Life of this patent
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Abstract
A cell culture tool configured to be produced in a simple facility to obtain a cell mass having a desired shape. The cell culture tool includes a cell culture base layer that contains a cell culture base. The cell culture base layer has a cell adhesive area to which a cell is adherable and a cell adhesion inhibitory area where cell adhesion is inhibited. The cell adhesion inhibitory area includes a modified product of the cell culture base.
Claims14
A cell culture tool including:
- a vessel including a flat bottom surface,
- a cell culture base layer that contains a cell culture base, and
- a photo-thermal conversion layer configured to convert light into heat,
- wherein
- the photo-thermal conversion layer is disposed on the flat bottom surface,
- the cell culture base layer is disposed on the photo-thermal conversion layer,
- a cell is not disposed on the cell culture base layer,
- the cell culture base layer has a cell adhesive area to which a cell is adherable and a cell adhesion inhibitory area where cell adhesion is inhibited,
- the cell adhesion inhibitory area includes a heat-denatured product of the cell culture base,
- the cell culture base is an extracellular matrix, and
- the flat bottom surface comprises a single flat bottom surface, an entirety of the vessel having the single flat bottom surface.
8 more elements
A method for producing a cell culture tool, the method comprising:
- an inhibitory area forming step of irradiating a cell culture tool including a cell culture base layer that includes a cell culture base with light to heat-denature the cell culture base and form a cell adhesion inhibitory area where cell adhesion is inhibited,
- wherein
- the cell culture tool includes a vessel including a flat bottom surface and a photo-thermal conversion layer configured to convert light into heat,
- the photo-thermal conversion layer is disposed on the flat bottom surface,
- the cell culture base layer is disposed on the photo-thermal conversion layer,
- a cell is not disposed on the cell culture base layer,
- in the inhibitory area forming step, the photo-thermal conversion layer is irradiated with the light,
- the cell culture base is an extracellular matrix, and
- the flat bottom surface comprises a single flat bottom surface, an entirety of the vessel having the single flat bottom surface.
6 more elements
Dependencies are inferred from the claim text.