Bispecific chimeric antigen receptors and encoding polynucleotides, vectors and cells thereof
AssigneeSeattle Children's Hospital
InventorMichael Jensen
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Abstract
The invention is directed to a bispecific chimeric antigen receptor, comprising: (a) at least two antigen-specific targeting regions; (b) an extracellular spacer domain; (c) a transmembrane domain; (d) at least one co-stimulatory domain; and (e) an intracellular signaling domain, wherein each antigen-specific targeting region comprises an antigen-specific single chain Fv (scFv) fragment, and binds a different antigen, and wherein the bispecific chimeric antigen receptor is co-expressed with a therapeutic control. The invention also provides methods and uses of the bispecific chimeric antigen receptors.
Claims30
A bispecific chimeric antigen receptor (CAR), comprising, in N-terminal to C-terminal order:
- a. an anti-CD20 scFv comprising a VH and VL, wherein the VH and VL amino acid sequences comprise the anti-CD20 VH and VL amino acid sequences, respectively, in SEQ ID NO:
- 12;
- b. an anti-CD19 scFv comprising a VH and VL, wherein the VH and VL amino acid sequences comprise the anti-CD19 VH and VL amino acid sequences, respectively, in SEQ ID NO:
- 12;
- c. a CD8α hinge extracellular spacer domain;
- d. a CD8α transmembrane domain;
- e. a 4-1BB co-stimulatory domain;
- and
- f. a CD3 zeta intracellular signaling domain.
Dependencies are inferred from the claim text.