Method of manufacturing diabetic foot patient-specific dermal regeneration sheet and diabetic foot patient-specific dermal regeneration sheet manufactured using the same

A method of manufacturing a diabetic foot patient-specific skin regeneration sheet, comprising:

• A) extracting autologous adipose tissue;
• B) removing fibers in the autologous adipose tissue using a first filter having a pore diameter of 2 to 3 mm, retaining the fibers on the first filter and passing the autologous adipose tissue through the first filter;
• C) crushing the autologous adipose tissue from which the fibers are removed by sequentially passing it through a second filter having a pore diameter of 450 to 550 μm and a third filter having a pore diameter of 150 to 250 μm, producing a crushed autologous adipose tissue;
• D) acquiring an adipose tissue extract by filtrating the crushed autologous adipose tissue mixed with a saline solution by a fourth filter having a pore diameter of 25 to 75 μm, acquiring a material collected by the fourth filter to be used as adipose tissue extract, and disposing of the filtrate;
• E) obtaining 3D data of a skin defect area of a patient using a 3D scanner, and then based on the data, manufacturing a 3D mold corresponding to the skin defect area using a 3D printer;
• F) forming a proto-layer by applying a first solution containing the adipose tissue extract and fibrinogen to the inside of the 3D mold;
• G) curing the proto-layer into a skin regeneration layer by applying a second solution containing thrombin onto the proto-layer;
• and
• H) removing the 3D mold;
• wherein the volume ratio of the adipose tissue extract and the fibrinogen in the first solution is 3:1 to 5:1.