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US 12,465,573 B2Granted
Structured nanocoatings for the stabilization of pluripotent stem cell media components
AssigneeWisconsin Alumni Research Foundation
InventorsWilliam L. Murphy, Andrew Khalil, Angela Xie, Hunter Johnson
Life of this patent
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Abstract
Disclosed are compositions and methods for pluripotent stem cell culture. Compositions include mineral coated microparticles having a core and a mineral coating, wherein the mineral coating includes fibroblast growth factor. Also disclosed are methods for pluripotent stem cell culture methods using mineral coated microparticles including fibroblast growth factor.
Claims8
A pluripotent stem cell culture method for maintaining stem cell pluripotency, the method comprising:
- contacting a pluripotent stem cell with a mineral coated microparticle, wherein the mineral coated microparticle comprises a core and a mineral coating that comprises fibroblast growth factor in an amount of fibroblast growth factor ranging from about 0.1 ng fibroblast growth factor per mg mineral coated microparticle to about 10,000 ng fibroblast growth factor per mg mineral coated microparticle, and wherein stem cell pluripotency is maintained when a minimum of 95% Oct4+/Nanog+ cell population is maintained at each cell passage.
Dependencies are inferred from the claim text.