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US 12,461,106 B2Granted
Ultra bright dimeric or polymeric dyes with spacing linker groups
AssigneeSony Group Corporation
InventorsTracy Matray, Sharat Singh
Life of this patent
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Abstract
Compounds useful as fluorescent or colored dyes are disclosed. The compounds have the following structure (I): or a stereoisomer, tautomer or salt thereof, wherein R1, R2, R3, R4, R5, L1, L2, L3, L4, M, m and n are as defined herein. Methods associated with preparation and use of such compounds are also provided.
Claims19
A compound having the following structure (IA):
- or a stereoisomer, salt or tautomer thereof, wherein:
- M is, at each occurrence, independently a fluorescent dye, wherein at least one occurrence of M has absorbance for use in fluorescence resonance energy transfer (FRET), and at least one occurrence of M has emissions for use in FRET;
- L1 is at each occurrence, independently either i) an alkylene linker;
- ii) a heteroalkylene linker including at least one nitrogen atom;
- or iii) a linker comprising a triazolyl functional group;
- L2 and L3 are, at each occurrence, independently an alkylene or heteroalkylene linker;
- R1 is, at each occurrence, H;
- R2 is Q, or a protected form thereof, or a linker comprising a covalent bond to Q;
- R3 comprises a deoxythymidine (dT) group;
- R4 is, at each occurrence, OH, O− or ORd;
- R5 is, at each occurrence, oxo;
- Rd is a counter ion;
- Q is a moiety comprising a sulfhydryl, disulfide, activated ester, isothiocyanate, azide, alkyne, alkene, diene, dienophile, acid halide, sulfonyl halide, phosphine, «-haloamide, biotin, amino or maleimide functional group, wherein the activated ester is an N-succinimide ester, imidoester or polyflourophenyl ester, and wherein the azide is an alkyl azide or acyl azide;
- m is, at each occurrence, independently an integer of one to 10;
- n is an integer of one to 32;
- and
- z is an integer from 2 to 25.
14 more elements
Dependencies are inferred from the claim text.