Compositions and methods for amplifying or detecting varicella-zoster virus
AssigneeGen-Probe Incorporated
InventorsMarcela Alejandra Carvallo Pinto, Amber Jean Hillius, Ankur Shah
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Abstract
Disclosed are oligonucleotides, oligonucleotide compositions, kits, methods, formulations, and reaction mixtures that provide for sensitive and specific detection of a target nucleic acid sequence, or amplicon generated from a target nucleic acid sequence, of Varicella-Zoster Virus (VZV1 (if present) in a sample. The oligonucleotides, compositions, kits, methods, formulations, and reaction mixtures can be used to detect the presence of VZV in a sample. The oligonucleotides, compositions, kits, methods, formulations, and reaction mixtures can also be used to amplify specific target nucleic acid regions of VZV.
Claims15
A composition for amplifying a Varicella-Zoster Virus (VZV) target nucleic acid sequence and detecting an amplified VZV target nucleic acid sequence comprising:
- (a) a forward amplification primer 19-50 nucleobases in length and comprising the nucleobase sequence of SEQ ID NO:
- 1, 2, 3, 4, 5, 6, or 7;
- and
- (b) a reverse amplification primer 19-50 nucleobases in length and comprising the nucleobase sequence of SEQ ID NO:
- 16, 17, 18, 19, 20, 21, or 22, wherein:
- (i) (a) comprises SEQ ID NO:
- 1 and (b) comprises SEQ ID NO:
- 16 or 17;
- (ii) (a) comprises SEQ ID NO:
- 2 and (b) comprises SEQ ID NO:
- 17;
- (iii) (a) comprises SEQ ID NO:
- 3 and (b) comprises SEQ ID NO:
- 18;
- (iv) (a) comprises SEQ ID NO:
- 4 and (b) comprises SEQ ID NO:
- 19;
- (v) (a) comprises SEQ ID NO:
- 5 and (b) comprises SEQ ID NO:
- 20;
- (vi) (a) comprises SEQ ID NO:
- 6 and (b) comprises SEQ ID NO:
- 21;
- or
- (vii) (a) comprises SEQ ID NO:
- 7 and (b) comprises SEQ ID NO:
- 22;
- and
- (c) a detection probe comprising a detectable label.
Dependencies are inferred from the claim text.