Methods for analyzing viral nucleic acid
AssigneeGrail, LLC
InventorsYuk-Ming Dennis Lo, Kwan Chee Chan, Weng In Chu
Life of this patent
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Abstract
Provided herein are methods, systems, and computer readable medium for detecting nucleic acid from a pathogen, e.g., virus, e.g., Epstein-Barr virus (EBV), in a cell-free nucleic sample from an individual at risk of a condition, e.g., nasopharyngeal carcinoma (NPC). The methods, systems, and computer readable medium can be used to screen for the presence of the condition, e.g., NPC, using thresholds adjusted based on sample attributes.
Claims28
A method of screening and treating a subject for a tumor, the method comprising selecting a subject, and based on said selecting, administering a treatment to the subject effective to treat a tumor in the subject, wherein:
- a) the selecting comprises (i) receiving results of an assay of a biological sample of the subject measuring an amount of cell-free nucleic acid from an Epstein-Barr Virus (EBV) in the biological sample, and (ii) identifying the amount of EBV cell-free nucleic acid in the biological sample as being above a threshold amount;
- b) the threshold amount distinguishes reference biological samples of reference subjects with a known tumor from reference biological samples of reference subjects without the known tumor;
- c) the subject and the reference subjects are characterized by one or more of the following:
- (i) age above 45 years old;
- (ii) smoking status is a smoker;
- or
- (iii) mean outdoor ambient temperature on a day the respective biological samples were collected at a location within 50 km of a location at which the respective biological samples were collected is below 30° C.;
- and
- d) the treatment is selected from the group consisting of:
- chemotherapy, radiation therapy, surgery, and antibody therapy.
Dependencies are inferred from the claim text.