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US 12,460,261 B2Granted
Compositions and methods for nucleic acid sequencing
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. Glezer, Mohammad Vatankhah Varnosfaderani, Daan Witters, Vahid Karimkhani
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Abstract
Provided herein are methods and compositions for improved sequencing techniques using, for example, polymeric particles and/or three-dimensional structures.
Claims20
A method of detecting polynucleotides in a polymer scaffold, the method comprising:
- (a) amplifying polynucleotides to produce discrete amplicon clusters within a three-dimensional polymer scaffold, wherein the amplicons are covalently bound to the polymer scaffold;
- (b) contacting the polymer scaffold with a plurality of fluorescently labeled molecules and, within two or more discrete amplicon clusters, binding a fluorescently labeled molecule to a nucleic acid molecule in the amplicon cluster;
- (c) detecting the fluorescently labeled molecules to identify the polynucleotide in the polymer scaffold.
Dependencies are inferred from the claim text.