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US 12,460,238 B2Granted
Methods of producing compositions comprising hydrophilic sophorolipids
AssigneeLocus Solutions Ipco, LLC
InventorsSean Farmer, Ken Alibek, Amir Mahmoudkhani, Janaina Izabel Da Silva De Aguiar
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Abstract
The subject invention provides materials and methods for producing high-HLB sophorolipid-based compositions and/or for increasing the water solubility of sophorolipid compositions. More specifically, the subject invention provides for the production and post-fermentation alteration of SLP molecules, which can be used for formulating a variety of useful compositions.
Claims8
A method for producing a sophorolipid composition with an HLB of 9 or greater, the method comprising:
- obtaining a yeast fermentation product comprising a mixture of hydrophilic SLP molecules comprising non-acetylated linear SLP, and hydrophobic SLP molecules comprising lactonic SLP and mono-and di-acetylated linear SLP, said mixture being produced by cultivating a SLP-producing microorganism;
- functionalizing the hydrophobic SLP molecules by converting them into hydrophilic molecules to produce a hydrophilic SLP fraction comprising only hydrophilic SLP molecules, wherein the hydrophobic SLP molecules are converted to hydrophilic SLP molecules by mixing the hydrophobic SLP molecules with a base;
- producing a purified hydrophobic SLP fraction;
- and
- mixing the hydrophilic SLP fraction with the purified hydrophobic SLP fraction at a ratio of hydrophilic SLP molecules to purified hydrophobic SLP molecules between 60:40 to 75:25.
2 more elements
Dependencies are inferred from the claim text.