Method for the production of high levels of PUFA in plants
AssigneeBASF Plant Science GmbH
InventorsToralf Senger, Hui Yang, Carl Andre, Patricia Vrinten
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Abstract
The present invention is concerned with materials and methods for the production of genetically modified plants, particularly where the plants are for the production of at least one unsaturated or polyunsaturated fatty acid. The invention is also concerned with identification of genes conveying an unsaturated fatty acid metabolic property to a plant or plant cell, and generally relates to the field of phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT).
Claims5
A method for increasing the delta-6 elongase conversion efficiency in an oil crop plant of a Brassica species that produces very long chain polyunsaturated fatty acids (vlcPUFAs) and expresses a delta-6 elongase, comprising:
- providing an oil crop plant of a Brassica species that expresses a delta-6 elongase and a delta-6 desaturase and that has decreased activity, compared to a control plant, of one or more phosphotidylcholine:diacylglycerol cholinephosphotransferase (PDCT) selected from the group consisting of:
- (a) PDCT3 and/or PDCT5 having at least 80% sequence identity with SEQ ID NO:
- 18, 20, 22, 24, 26, 28, 30, 32, 50, 52, 54, 56, 58, and/or 60;
- (b) PDCT3 and/or PDCT5 encoded by a polynucleotide having at least 80% sequence identity with SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59;
- and
- (c) PDCT3 and/or PDCT5 encoded by a polynucleotide that differs from SEQ ID NO:
- 17, 19, 21, 23, 27, 29, 31, 49, 51, 53, 55, 57 and/or 59 due to the degeneracy of the genetic code
- wherein the plant produces docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and docosapentaenoic acid (DPA).
Dependencies are inferred from the claim text.