Phage-derived particles for in situ delivery of DNA payload into C. acnes population
AssigneeEligo Bioscience
InventorsAymeric Leveau, Inès Canadas Blasco, Aurélie Mathieu, Antoine Decrulle
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Abstract
The invention relates to C. acnes carrying DNA vectors with a C. acnes phage packaging signal and a gene of interest. The invention encompasses a C. acnes producer cell carrying DNA vectors, with a C. acnes phage packaging signal and a gene of interest, for the production of phage-derived particles that can robustly transduce C. acnes receiver cell allowing transgene expression. The invention encompasses C. acnes phage-derived particles carrying these vectors, C. acnes containing these vectors or modified by transduction of these phage-derived particles, and methods of using these phage-derived particles.
Claims17
A C. acnes phage-derived particle comprising a phagemid lacking genes encoding phage structural proteins, said phagemid comprising:
- a phage packaging signal allowing packaging of the phagemid in a Cutibacterium acnes phage capsid,
- wherein the phage packaging signal is at least 87% identical to SEQ ID NO:
- 76,
- a gene of interest, and
- a CRISPR-Cas system.
A phagemid lacking genes encoding phage structural proteins comprising:
- a phage packaging signal allowing packaging of the phagemid in a Cutibacterium acnes phage capsid,
- wherein the phage packaging signal is at least 87% identical to SEQ ID NO:
- 76,
- a gene of interest, and
- a CRISPR-Cas system.
Dependencies are inferred from the claim text.