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US 12,460,204 B2Granted
Artificial nucleic acid molecules for improved protein or peptide expression
AssigneeCureVac AG
InventorAndreas Thess
Life of this patent
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Abstract
The invention relates to an artificial nucleic acid molecule comprising at least one 5′ UTR element which is derived from a TOP gene, at least one open reading frame, and preferably at least one histone stem-loop. Optionally the artificial nucleic acid molecule may further comprise, e.g. a poly(A)sequence, a poyladenylation signal, and/or a 3′ UTR. The invention further relates to the use of such an artificial nucleic acid molecule in gene therapy and/or genetic vaccination.
Claims33
An artificial nucleic acid molecule selected from the group consisting of
- (i) a DNA molecule comprising, from 5′ to 3′:
- a) at least one 5-untranslated region element (5′ UTR element) which comprises a nucleic acid sequence having an identity of at least 90% to the nucleic acid sequence according to SEQ ID NO:
- 1455;
- and
- b) at least one open reading frame (ORF), wherein the ORF is heterologous relative to the 5′ UTR element;
- and
- (ii) a RNA molecule encoded by the DNA molecule.
4 more elements
An artificial RNA molecule comprising, from 5′ to 3′:
- i) a 5′-cap structure;
- ii) at least one 5′-untranslated region element (5′ UTR element) which comprises a nucleic acid sequence having an identity of at least 90% to the RNA sequence encoded by the nucleic acid sequence according to SEQ ID NO:
- 1455;
- and
- iii) at least one open reading frame (ORF), wherein the ORF is heterologous relative to the 5′ UTR element.
2 more elements
Dependencies are inferred from the claim text.