Modular polypeptide libraries and methods of making and using same
AssigneeThe Regents of the University of California
InventorsWendell A. Lim, Scott M. Coyle, Russell M. Gordley, Kole T. Roybal
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Abstract
The present disclosure provides synthetic modular polypeptide libraries and nucleic acids encoding such synthetic modular polypeptide libraries. Also provided are methods of making synthetic modular polypeptide libraries and nucleic acids encoding synthetic modular polypeptide libraries. Methods of screening a synthetic modular polypeptide library to identify a selected phenotype associated with a member of a synthetic modular polypeptide library are also provided where such methods find use in both in vitro and in vivo assays.
Claims10
A barcoded library of nucleic acids, wherein:
- (i) the members of the library each comprise a barcode region and a coding sequence that is operably linked to a promoter;
- (ii) within each member, the barcode region is separate from the coding sequence;
- (iii) the coding sequence encodes a modular protein having at least a first domain, a second domain, and a third domain;
- (iv) the sequences of the first domain, the second domain and the third domain independently vary in the library;
- (v) the barcode region comprises at least a first barcode, a second barcode and a third barcode, and
- (vi) within each member, the first barcode, the second barcode and the third barcode correspond to and identify the sequence of the first domain, the sequence of the second domain, and the sequence of the third domain of the modular protein, respectively.
Dependencies are inferred from the claim text.