Esterases and uses thereof
AssigneeCarbios
InventorVincent Tournier
Life of this patent
Estimating the term…
Abstract
The present invention relates to novel esterases, more particularly to esterase variants having improved activity and/or improved thermostability compared to the esterase of SEQ ID NO: 1 and the uses thereof for degrading polyester containing material, such as plastic products. The esterases of the invention are particularly suited to degrade polyethylene terephthalate, and material containing polyethylene terephthalate.
Claims30
An esterase which (i) has at least 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, and (ii) has at least one amino acid substitution selected from F209I/G/H/L/R/T, D12F/Y/R, T50P, T63Q, S66H, W69R, T89R/F, D94S, S121W, T153A, N158Q, T168Q, P180E, A182R, F188I/Y, S197P, E202M, G205C/K, T207L, P211A, N212D/Q, K216P, K220E, Q238T, L240A, P242K, G243Y, P244N, G247A/D/E/H/S, G250C/Y and P260S wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1, (iii) has a polyester degrading activity, and (iv) has an increased thermostability and/or an increased degrading activity compared to the esterase of SEQ ID NO:
- 1.
An esterase which (i) has at least 80%, 85%, 90%, 95%, 96%, 97%, 98% or 99% identity to the full length amino acid sequence set forth in SEQ ID NO:
- 1, and (ii) has at least three amino acid substitutions at positions selected from F209, T61, A65, Q92, G135, T168, T177, T183, D204, N212 and E253, wherein the positions are numbered by reference to the amino acid sequence set forth in SEQ ID NO:
- 1, (iii) has a polyester degrading activity, and (iv) has an increased thermostability and/or an increased degrading activity compared to the esterase of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.