Second additional phytase variants and methods
AssigneeFornia BioSolutions, Inc.
InventorsJie Yang, Xiyun Zhang, Yangyuan Li, Jiang Huang
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Abstract
The present invention relates to variant phytase enzymes and their use thereof.
Claims42
A composition comprising a variant phytase enzyme comprising an amino acid substitution at position 285 as compared to SEQ ID NO:7, wherein said variant phytase enzyme has at least 85% sequence identity to SEQ ID NO:7 and is not SEQ ID NO:1;
- and wherein said variant phytase enzyme has phytase activity.
A composition comprising a variant phytase enzyme comprising an amino acid substitution at position 285 as compared to SEQ ID NO:7, wherein said variant phytase enzyme has at least 2-fold increase in residual activity as compared to SEQ ID NO:7 under a condition selected from the group consisting of thermochallenge at about 75° C., thermochallenge at about 80° C., thermochallenge at about 85° C., and thermochallenge at about 90° C.;
- and wherein said variant phytase enzyme has at least 85% sequence identity to SEQ ID NO:7 and is not SEQ ID NO:1.
A composition comprising a variant phytase enzyme comprising one or more amino acid substitutions as compared to SEQ ID NO:7,
- wherein said one or more amino acid substitutions occur at positions corresponding to positions selected from the group consisting of positions 4, 7, 25, 35, 55, 60, 67, 69, 73, 74, 75, 116, 117, 118, 119, 120, 121, 136, 137, 157, 159, 173, 192, 212, 238, 255, 276, 277, 280, 281, 282, 283, 284, 285, 286, 287, 288, 315, 346, 354, 364, 378, 380, 381, and 403 of SEQ ID NO:7,
- wherein said variant phytase enzyme has at least 85% sequence identity to SEQ ID NO:7 and is not SEQ ID NO:1;
- and wherein said variant phytase enzyme has phytase activity, and
- wherein said variant phytase enzyme has at least 10-fold increase in residual activity as compared to SEQ ID NO:
- 7 under a condition selected from the group consisting of thermochallenge at about 75° C., thermochallenge at about 80° C., thermochallenge at about 85° C., and thermochallenge at about 90° C.
A composition comprising a variant phytase enzyme comprising an amino acid substitution at position 285 as compared to SEQ ID NO:7,
- wherein said variant phytase enzyme has at least 85% sequence identity to SEQ ID NO:7 and is not SEQ ID NO:1;
- and wherein said variant phytase enzyme has phytase activity, and
- wherein said variant phytase enzyme comprises one or more amino acid substitutions as compared to SEQ ID NO:1 and has at least 90% sequence identity to SEQ ID NO:1.
A composition comprising a variant phytase enzyme comprising one or more amino acid substitutions as compared to SEQ ID NO:7,
- wherein said one or more amino acid substitutions occur at positions corresponding to positions selected from the group consisting of positions 4, 7, 25, 35, 55, 60, 67, 69, 73, 74, 75, 116, 117, 118, 119, 120, 121, 136, 137, 157, 159, 173, 192, 212, 238, 255, 276, 277, 280, 281, 282, 283, 284, 285, 286, 287, 288, 315, 346, 354, 364, 378, 380, 381, and 403 of SEQ ID NO:7,
- wherein said variant phytase enzyme has at least 85% sequence identity to SEQ ID NO:7 and is not SEQ ID NO:1;
- and wherein said variant phytase enzyme has phytase activity, and
- wherein said variant phytase enzyme has at least 2% increase in residual activity as compared to SEQ ID NO:1 under a condition selected from the group consisting of thermochallenge at about 75° C., thermochallenge at about 80° C., thermochallenge at about 85° C., and thermochallenge at about 90° C.
Dependencies are inferred from the claim text.