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US 12,460,181 B2Granted
Membrane coverage and orientation and methods, compositions, and kits related thereto
AssigneeGeorge Mason University
InventorsBarney Bishop, Mrinalini Ramanan
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Abstract
Cells and lipid-based structures are described herein, in particular cells and lipid-based structures comprising membrane portions derived from cells having a label covalently attached thereto, as are compositions and kits comprising such cells and structures, and methods for using the cells and structures, in particular for determining membrane coverage and/or orientation as well as for screening methods/assays, and the like.
Claims5
A lipid-based structure comprising a lipid bilayer having a membrane portion derived from a plasma membrane of a cell and comprising a protein,
- wherein the lipid bilayer isolates an interior space of the structure from an external environment,
- wherein the protein of the membrane portion has the same orientation found on the plasma membrane of the cell with the protein,
- wherein the protein has an extracellular domain covalently attached to a label, and
- wherein the label is a fluorescent label.
1 more element
Dependencies are inferred from the claim text.