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US 12,460,179 B2Granted
Lyophilized compositions containing umbilical cord lining stem cells conditioned medium
AssigneeRestem LLC
InventorsRafael Gonzalez, Toai Nguyen
Life of this patent
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Abstract
Factor rich compositions produced from umbilical cord (UC) mesenchymal stem cells (MSCs) are described. Secretory UC MSCs in serum free culture conditions produce a factor rich conditioned medium which may be concentrated and filtered to obtain clinical grade products.
Claims6
A lyophilized factor rich composition derived from umbilical cord lining stem cells (ULSCs) conditioned medium comprising:
- a) a medium conditioned by ULSCs at a confluence of 80 to 90% without serum, lipids, exogenous growth factors, dexamethasone or antibiotics but including L-alanyl-L-glutamine;
- b) a growth factor selected from the group consisting of stem cell factor (SCF), vascular endothelial growth factor (VEGF), nerve growth factor (NGF) and combinations thereof;
- c) a cytokine or chemokine selected from the group consisting of interferon gamma (IFN-γ), interleukins (IL) IL-3, IL-4, IL-6, IL-7, IL-8, IL-18, macrophage inflammatory protein-1 beta (MIP1B), monocyte chemoattractant protein-1 (MCP1) and tumor necrosis factor alpha (TNFα) and combinations thereof;
- d) a glycosaminoglycan (GAG) selected from the group consisting of hyaluronic acid (HA) and chondroitin sulfate (CS) and combinations thereof;
- and
- e) a collagen selected from the group consisting of collagen I and collagen III.
3 more elements
Dependencies are inferred from the claim text.