Method of inducing or improving wound healing properties of mesenchymal stem cells
AssigneeCellresearch Corporation Pte. Ltd.
InventorsToan Thang Phan, Gavin Tan
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Abstract
The present invention relates to a method of inducing or improving wound healing properties of a mesenchymal stem cell population, the method comprising cultivating the mesenchymal stem cell population in a culture medium comprising DMEM (Dulbecco's modified eagle medium), F12 (Ham's F12 Medium), M171 (Medium 171) and FBS (Fetal Bovine Serum). The invention also relates to a mesenchymal stem population, wherein at least about 90% or more cells of the stem cell population express each of the following markers: CD73, CD90 and CD105 and lack expression of the following markers: CD34, CD45 and HLA-DR. The invention also relates to a pharmaceutical composition of this mesenchymal stem population.
Claims10
A method of inducing or improving wound healing properties of a mesenchymal stem cell population derived from umbilical cord, the method comprising cultivating the mesenchymal stem cell population in a culture medium comprising 55 to 65% (v/v) Dulbecco's modified Eagle's medium (DMEM), 5 to 15% (v/v) Ham's F12 Medium (F12), 15 to 30% (v/v) Medium 171 (M171) and 1 to 8% (v/v) Fetal Bovine Serum (FBS) in a bioreactor under conditions such that the mesenchymal stem cell population in said culture medium results in an increase in expression and/or secretion of at least three of Angiopoietin 1 (Ang-1), TGF-β1, VEGF, and HGF by the mesenchymal stem cell population relative to cultivation of said mesenchymal stem cell population in a reference culture medium that does not comprise all of DMEM, F12, M171, and FBS.
Dependencies are inferred from the claim text.