US 12,460,177 B2 · Composition for promoting efficiency of somatic cell replication

Abstract

The present invention relates to a composition for promoting the efficiency of somatic cell replication, comprising endothelin as an active ingredient. When somatic cells are treated with the composition of the present invention, H3K9me3, which is suspected to be a factor for the lowering success rate of nuclear substitution, is inhibited, and H3K4me3, which has the opposite effect, is increased to increase the success rate of nuclear substitution. Accordingly, the composition of the present invention increases the efficiency of somatic cell replication, thereby increasing the efficiency of producing pluripotent stem cells derived from nuclear substitution, and ultimately, the composition is expected to contribute to the development of cell therapeutic agents.

Claims4

Dependent claims

A method of promoting somatic cell nuclear transfer efficiency, comprising:

a) contacting a donor cell with a composition comprising endothelin as an active ingredient, wherein the donor cell is a mesenchymal stem cell;
and
b) extracting the nucleus of the donor cell, wherein the endothelin induces a transition of H3K9me3 to H3K4me3 in the Apobec1 promoter region of the extracted nucleus, thereby enhancing the efficiency of somatic cell nuclear transfer when the nucleus extracted in step b) thereby promoting blastocyst formation efficiency is transferred to an oocyte.

Dependencies are inferred from the claim text.

Facts

Application

16/969,045Filed 12 Feb 2019

Priority

KR 10-2018-001747213 Feb 2018

Published

US 2021/0139845 A113 May 2021

Granted

4 Nov 2025

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

C12N 5/0604
Embryonic cells ; Embryoid bodies: whole embryos; Culture medium therefor
C07K 14/57536
Hormones: endothelin, vasoactive intestinal contractor [VIC]
C12N 15/877
Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos: techniques for…
C12N 2501/998
Active agents used in cell culture processes, e.g. differentation: proteins not provided for elsewhere

Composition for promoting efficiency of somatic cell replication

Life of this patent

Composition for promoting efficiency of somatic cell replication

Life of this patent

Abstract

Claims4