Cell culture system with controlled gas transfer boundary conditions

A cell culture system comprising:

• a manifold;
• and,
• a plurality of cell culture modules each independently and releasably coupled to at least one of a plurality of coupling sites of the manifold, each cell culture module of the plurality of cell culture modules comprising:
• a chamber configured to hold a nutrient capacitive medium;
• a cell growth surface configured to support a cell culture disposed within the nutrient capacitive medium;
• a plurality of gas transfer interfaces spatially distributed in relation to the cell growth surface and configured such that the nutrient capacitive medium is in gaseous communication with a controlled gas source through the plurality of gas transfer interfaces, each of the plurality of gas transfer interface comprising:
• a plenum in gas communication with the manifold;
• and,
• a gas permeable membrane separating the nutrient capacitive medium and the plenum,
• wherein the plurality of gas transfer interfaces are disposed at least on sides and on a top of the cell culture such that a concentration of at least one target gas in the nutrient capacitive medium is selectively controlled by maintaining predetermined boundary conditions of the cell growth surface via the plurality of gas transfer interfaces.