Monoclonal antibodies for prevention and treatment of herpes simplex viral infections
AssigneesDuke University, Albert Einstein College of Medicine
InventorsBetsy C. Herold, Garnett Kelsoe, Masayuki Kuraoka, Clare Burn, William R. Jacobs, Jr.
Life of this patent
Estimating the term…
Abstract
Provided are antibodies and antigen-binding fragments which bind to herpes simplex virus-2 (HSV-2), methods of use employing the antibodies and/or antigen-binding fragments, and pharmaceutical compositions comprising the antibodies and/or antigen-binding fragments.
Claims27
An antibody or antigen-binding fragment, comprising:
- a heavy chain variable region comprising GYSFTTYD (SEQ ID NO:1), IYPREGST (SEQ ID NO:2), and ATYGSSRYYTMDY (SEQ ID NO:3);
- and/er a light chain variable region comprising ESVDNFGISF (SEQ ID NO:4), AAS (SEQ ID NO:5), and QQSKEVPLT (SEQ ID NO:6), or
- a heavy chain variable region comprising GYSITNGNH (SEQ ID NO:7), IRSSGSS (SEQ ID NO:8), and ARGGGLRHYFDY (SEQ ID NO:9);
- and/er a light chain variable region comprising GNIHNY (SEQ ID NO:10), HAE (SEQ ID NO:11), and QHFWSTPYT (SEQ ID NO:12), or
- a heavy chain variable region comprising GFTFTDYY (SEQ ID NO:13), IRNKANGYTT (SEQ ID NO:14), and ACGNYVGYAMDY (SEQ ID NO:15);
- and/er a light chain variable region comprising QSLLNSRTRKNY (SEQ ID NO:16), WAS (SEQ ID NO:17), and KQSYNLYT (SEQ ID NO:18), or
- a heavy chain variable region comprising GFSLSRHD (SEQ ID NO:79), IWGDGST (SEQ ID NO:80), and AKEDYGIFPY (SEQ ID NO:81);
- and/er a light chain variable region comprising QDISSY (SEQ ID NO:82), RAN (SEQ ID NO:83), and LQYDEFPLT (SEQ ID NO:84), or
- a heavy chain variable region comprising GYTFTNYD (SEQ ID NO:87), IYPRDGST (SEQ ID NO:88), and ARGIFYVNYDVY (SEQ ID NO:89);
- and/er a light chain variable region comprising DHINNW (SEQ ID NO:90), GAA (SEQ ID NO:91), and QQYWSSPLT (SEQ ID NO:92), or
- a heavy chain variable region comprising
- GFSLNNYD (SEQ ID NO:
- 85),
- IWGDGST (SEQ ID NO:
- 80), and AKEDYGIFPY (SEQ ID NO:81);
- and a light chain variable region comprising
- QDINSY (SEQ ID NO:86), RAN (SEQ ID NO:83), and
- LOYDEFPLT (SEQ ID NO:84);
- or,
- a heavy chain variable region comprising EYEFPSHD (SEQ ID NO:93), INSDGGST (SEQ ID NO:94), and ARHSSGYVLDY (SEQ ID NO:95);
- and a light chain variable region comprising DHINHW (SEQ ID NO:96), GAT (SEQ ID NO:97), and QQYWSTPYT (SEQ ID NO:98).
A nucleic acid encoding a heavy chain variable region of an antibody, wherein the heavy chain variable region has an amino acid sequence comprising:
- (SEQ ID NO:
- 1)
- GYSFTTYD,
- (SEQ ID NO:
- 2)
- IYPREGST,
- and
- (SEQ ID NO:
- 3)
- ATYGSSRYYTMDY;
- or
- (SEQ ID NO:
- 7)
- GYSITNGNH,
- (SEQ ID NO:
- 8)
- IRSSGSS,
- and
- (SEQ ID NO:
- 9)
- ARGGGLRHYFDY;
- or
- (SEQ ID NO:
- 13)
- GFTFTDYY,
- (SEQ ID NO:
- 14)
- IRNKANGYTT,
- and
- (SEQ ID NO:
- 15)
- ACGNYVGYAMDY;
- or
- (SEQ ID NO:
- 79)
- GFSLSRHD,
- (SEQ ID NO:
- 80)
- IWGDGST,
- and
- (SEQ ID NO:
- 81)
- AKEDYGIFPY;
- or
- (SEQ ID NO:
- 85)
- GFSLNNYD,
- (SEQ ID NO:
- 80)
- IWGDGST,
- and
- (SEQ ID NO:
- 81)
- AKEDYGIFPY;
- or
- (SEQ ID NO:
- 87)
- GYTFTNYD,
- (SEQ ID NO:
- 88)
- IYPRDGST,
- and
- (SEQ ID NO:
- 89)
- ARGIFYVNYDVY;
- or
- (SEQ ID NO:
- 93)
- EYEFPSHD,
- (SEQ ID NO:
- 94)
- INSDGGST,
- and
- (SEQ ID NO:
- 95)
- ARHSSGYVLDY.
A nucleic acid encoding a light chain variable region of an antibody, wherein the light chain variable region has an amino acid sequence comprising:
- (SEQ ID NO:
- 4)
- ESVDNFGISF,
- (SEQ ID NO:
- 5)
- AAS,
- and
- (SEQ ID NO:
- 6)
- QQSKEVPLT;
- or
- (SEQ ID NO:
- 10)
- GNIHNY,
- (SEQ ID NO:
- 11)
- HAE,
- and
- (SEQ ID NO:
- 12)
- QHFWSTPYT;
- or
- (SEQ ID NO:
- 16)
- QSLLNSRTRKNY,
- (SEQ ID NO:
- 17)
- WAS,
- and
- (SEQ ID NO:
- 18)
- KQSYNLYT;
- or
- (SEQ ID NO:
- 82)
- QDISSY,
- (SEQ ID NO:
- 83)
- RAN,
- and
- (SEQ ID NO:
- 84)
- LQYDEFPLT;
- or
- (SEQ ID NO:
- 86)
- QDINSY
- (SEQ ID NO:
- 83)
- RAN,
- and
- (SEQ ID NO:
- 84)
- LQYDEFPLT;
- or
- (SEQ ID NO:
- 90)
- DHINNW,
- (SEQ ID NO:
- 91)
- GAA,
- and
- (SEQ ID NO:
- 92)
- QQYWSSPLT;
- or
- (SEQ ID NO:
- 96)
- DHINHW,
- (SEQ ID NO:
- 97)
- GAT,
- and
- (SEQ ID NO:
- 98)
- QQYWSTPYT.
Dependencies are inferred from the claim text.