Centrifugal-driven microfluidic platform and method of use thereof

A centrifugal-driven microfluidic platform, comprising:

• a microfluidic module, comprising a microfluidic disk and a microfluidic structure;
• wherein the microfluidic structure further comprises:
• at least one injection chamber module, disposed on the microfluidic disk;
• wherein each of the at least one injection chamber module further comprises at least one transient pause chamber and at least one decanting chamber and at least one second eluent chamber, and the at least one decanting chamber is connected with the at least one second eluent chamber via a first valve;
• at least one separation column, disposed on the microfluidic disk and connected with the injection chamber module;
• at least one dispensing channel, disposed on the microfluidic disk and connected with the separation column by a connection channel module;
• a plurality of collection chambers, disposed on the microfluidic disk;
• wherein each of the collection chamber connects with the dispensing channel respectively;
• a waste chamber, disposed at the end of the dispensing channel;
• a driving module, wherein a microfluidic module is disposed on the driving module;
• an operation module, wherein the operation module drives the driving module;
• and
• a detection module, wherein the detection module connects with the microfluidic module and the driving module respectively;
• wherein at least one separation column is spiral shaped;
• wherein the at least one dispensing channel is spiral structure or arc shaped.