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AssigneesGenvor Inc., The United States of America as represented by the Secretary of Agriculture
InventorsJesse M. Jaynes, Kanniah Rajasekaran, Jeffrey W. Cary, Ronald Sayler, Rajtilak Majumdar
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Aspergillus flavus is an opportunistic, saprophytic fungus that infects maize and other fatty acid-rich food and feed crops and produces toxic and carcinogenic secondary metabolites known as aflatoxins. In vitro studies showed a five-fold increase in antifungal activity of AGM182 (vs. tachyplesin1) against A. flavus. Transgenic maize plants expressing AGM182 under maize Ubiquitin-1 promoter were produced through Agrobacterium-mediated transformation. PCR products confirmed integration of the AGM182 gene, while RT-PCR of maize RNA confirmed the presence of AGM182 transcripts. Maize kernel screening assay using a highly aflatoxigenic A. flavus strain (AF70) showed up to 72% reduction in fungal growth in the transgenic AGM182 seeds compared to isogenic negative control seeds.
A transgenic maize line expressing a synthetic AGM182 peptide, wherein the transgenic maize line is made by the method comprising the steps of:
A maize plant expressing a synthetic AGM182 peptide having the amino acid sequence of SEQ ID NO:2 that reduces aflatoxin production and fungal growth of Aspergillus Flavus.
A synthetic AGM182 peptide having the amino acid sequence of SEQ ID NO:2.
A vector comprising an gene, wherein the gene comprises a 129 bp nucleic acid sequence of SEQ ID NO:3.
A method of preventing or treating a plant to reduce growth of a mycotoxin producing fungal species comprising:
Dependencies are inferred from the claim text.